There are two major universities in Wenzhou: Wenzhou University and Wenzhou Medical College. Wenzhou University resulted from the merger of the former University of Wenzhou, Wenzhou Normal College and other various normal colleges in the rural towns of Wenzhou. Its main campus is situated in the University Town, Cha Shan. The former campus of Wenzhou Normal College on Xueyuan Road is still in use, while the former main campus of the University of Wenzhou now

serves as the campus of the Wenzhou Foreign Language School and the Second Experimental Middle School of Wenzhou (No.13 Middle School).

Wenzhou Medical College is a well-known college specializing in ophthalmology (national level key discipline), as well as the provision of other medical courses. Several of Wenzhou's major hospitals are affiliated to this college.